Comparison between attenuated and non-attenuated Eimeria vaccines. What is the difference?

20 March, 2018

Eimeria vaccines for poultry are mainly composed of live oocysts. Concerns about the safety of coccidiosis vaccines have been raised by field users. This study compares the safety parameters of commercial non-attenuated and attenuated Eimeria vaccines with EVALON®, a live attenuated coccidiosis vaccine for breeders and layers.

Most of the Eimeria vaccines available for chickens consist of live parasites that need to undergo two and sometimes three entire life cycles inside the host gut in order to trigger the immune system and subsequently establish a full protective immunity.

On the market, there are live non-attenuated and attenuated vaccines. Live non-attenuated vaccines consist of parasites that still maintain their natural virulence.

Control of the development of adverse reactions (coccidiosis disease) is achieved through the use of low numbers of oocysts in vaccine preparations and in some cases even by the use of anticoccidials to control the excessive spread of vaccine strains. Live attenuated vaccines are specifically designed to generate an immune response whilst limiting the threat of possible adverse events.

The most widely used attenuation system is the selection of strains by precocious development (1); although attenuation through passages on embryonated eggs has also been used in E.tenella for some commercial live attenuated vaccines. Therefore, there can be different degrees of attenuation due to the use of different attenuation processes. The aim of this study was to compare safety parameters of commercial non-attenuated and attenuated Eimeria vaccines with EVALON®, a live attenuated coccidiosis vaccine for breeders and layers.

Materials and methods

One-day-old COBB chicks were purchased from a commercial supplier in Spain. The weight of the birds at one day of age was between 40 g and 50 g. The animals were housed in experimental Eimeria-free facilities and raised in floor-pens with wood shavings as litter; the litter was not changed during the entire length of the study.

Temperature and humidity were checked daily and maintained at an average of 25º-26ºC and over 50%, respectively. Feed and water were provided ad libitum. Commercial feed contained neither antimicrobials nor anticoccidial additives. The animals were divided into 5 groups (1-5) of 105 birds each, using weight as a stratification factor. Table 1 shows the study design and the parameters evaluated (see Table 1).


No clinical signs were recorded in group 1, EVALON® and group 4 during the entire study. Mild clinical signs such as ruffled feathers and reduced activity were observed in groups 3 and 5 (non-attenuated vaccines). Moreover, group 5 also showed mild clinical signs such as pale legs and crest, slightly arched body and huddling. The duration of mild clinical signs was variable. These manifestations were detected in group 3 for 2 days, whereas in group 5 they lasted from 15 to 43 dpv.

Changes in the faeces were sporadically observed in EVALON® group and group 4, whereas in groups 3 and 5 they occurred almost continuously from the second-third week post vaccination.

There were no statistically significant changes in body weight at any time during the entire study (Mann-Whitney U test; p<0.05); however, at 37 dpv, the weights of control group 1 and EVALON® group were the greatest. These results are consistent with what was expected with EVALON®; since it is an attenuated vaccine, its replication is very fast with a peak at 21 dpv, leaving the digestive tract clear so that animals can continue to grow without interferences linked to the vaccine strains (Figure 1).

Figure 1. Body weights. Evaluated in dpv 21, 28 and 37. The values of each experimental group are presented as averages with standard deviation.

Throughout the study, EVALON® group did not show any statistically significant difference (Mann-Whitney U test; p<0.05) in average intestinal lesions compared to the control group (group 1). In contrast, statistically significant different average lesion scores (Mann-Whitney U test; p<0.05) were observed in different intestinal regions in groups 3 to 5 compared to the control group.

In particular, group 3 showed lesions in the rectum at 28 dpv. Group 4 showed lesions in the duodenum and jejunum at 28 dpv; in the rectum at 37 dpv. Group 5 showed lesions in the rectum at 28 dpv and in the caeca at 37 dpv. Figures 2 shows the Total Mean Lesion Score in each group.

The Total Mean Lesion score is the sum of the average lesion values for the different intestinal tracts in a number of birds (20 in this study).

Figure 2. Total Mean Lesion Score.

Eimeria oocysts were detected in the litter of all vaccinated groups; however, in group 4 their appearance was delayed compared to the other groups. The number of oocysts was higher in groups 3 and 5 compared to the other groups with the differences reaching 14.9 x 104 oocysts/g at day 7 (Figure 3).

However, after day 7, both groups 3 and 5 showed a significant decrease in the oocysts per gram (OPG) in the litter and this may be due to the fact that, under experimental conditions, the densities of the birds are lower compared to field conditions and this may affect the proper distribution of the vaccinal oocysts.

The EVALON® group behaved as expected in terms of OPGs: since it is an attenuated vaccine, replication never reached such high levels as the non-attenuated vaccines (group 3 and 5) and, in addition, the profile of the OPG curve shows the peak of the first replication at 7 dpv, with a 2nd peak at 21 dpv which corresponds to the second replication, after which the birds develop immunity against coccidiosis.

Figure 3. Number of oocysts in the litter samples. The results are presented by number of oocysts per gram of the litter sample obtained from pool analysis.


The results of this study show the presence of the following differences by product were shown in comparison with EVALON®:

  • Clinical signs and droppings alterations: more persistent in group 3 and 5, less in group 4.
  • Intestinal lesions: highest in groups 3, 4 and 5.
  • OPG in the litter: replication peaks of the vaccines very high at 7 dpv in groups 3 and 5, while in group 4 there was a delayed peak of replication of the oocysts at 21 dpv.

In conclusion, the results of this study indicate that both attenuation itself and its degree may be important factors that must be taken into account when choosing live Eimeria vaccines; the lack of attenuation and low degree of attenuation may partly justify the deterioration of the health of vaccinated animals.

On the other hand, this study confirms the non-interference of EVALON® in the productive parameters, as well as the absence of impact in the birds’ intestine. In addition to the durable protection inferred by EVALON®, these factors put the birds in a better position to achieve the objectives of uniformity and weight during the rearing period.


  • Jeffers T.K. (1975). Attenuation of Eimeria tenella through selection for precociousness. J Parasitol. Dec; 61(6):1083-90.

Related Posts


Check latests documents


Tools to prevent Eimeria


Facts you should know

Basic Personal Data Protection information:

Purposes: Managing the contractual and/or business relationship with HIPRA, including sending news, promotions and invitations to events sponsored by HIPRA.
Lawful basis: Performance of the contractual relationship and HIPRA's legitimate Interest.
Recipients: Third parties to which HIPRA has entrusted cloud computing, security, auditing, mailing, technical and computer support services, as well as companies in its group.
Rights: Request access to and rectification or erasure of personal data and other rights as explained in the additional information. You can seeview the detailed additional information about data protection in our Privacy Policy.

For further information, please check our detailed information on Data Protection.

Website for veterinarians

The contents of this website are aimed exclusively at prescribing veterinarians. By clicking on ACCEPT you state that you are a veterinary professional. In other case, please contact a veterinarian and click on DECLINE.